diff --git a/roles/configure_functional_tests/tasks/main.yaml b/roles/configure_functional_tests/tasks/main.yaml
index aa887114824..feb44c37244 100644
--- a/roles/configure_functional_tests/tasks/main.yaml
+++ b/roles/configure_functional_tests/tasks/main.yaml
@@ -25,6 +25,7 @@
         # This is DB USER used in mysql db
         DATABASE_USER=openstack_citest
         MYSQL_GATHER_PERFORMANCE={{ MYSQL_GATHER_PERFORMANCE | default(true) }}
+        PYTHON3_VERSION={{ PYTHON3_VERSION | default('3') }}
 
         source $NEUTRON_DIR/tools/configure_for_func_testing.sh
         if is_fedora; then
diff --git a/zuul.d/base.yaml b/zuul.d/base.yaml
index 59a13850899..73ce057352e 100644
--- a/zuul.d/base.yaml
+++ b/zuul.d/base.yaml
@@ -150,6 +150,10 @@
     vars:
       nslookup_target: 'opendev.org'
       enable_fips: True
+      # NOTE(ykarel) remove python3 override with 10-stream switch
+      PYTHON3_VERSION: 3.12
+      tox_environment:
+        TOX_PYTHON: python3.12
       devstack_localrc:
         ISCSI_CHAP_ALGORITHMS: SHA3-256,SHA256
         Q_BUILD_OVS_FROM_GIT: true
@@ -235,6 +239,10 @@
     vars:
       nslookup_target: 'opendev.org'
       enable_fips: True
+      # NOTE(ykarel) remove python3 override with 10-stream switch
+      PYTHON3_VERSION: 3.12
+      tox_environment:
+        TOX_PYTHON: python3.12
       devstack_localrc:
         ISCSI_CHAP_ALGORITHMS: SHA3-256,SHA256
         Q_BUILD_OVS_FROM_GIT: true
diff --git a/zuul.d/tempest-singlenode.yaml b/zuul.d/tempest-singlenode.yaml
index d2da0711a29..02a3ac0a938 100644
--- a/zuul.d/tempest-singlenode.yaml
+++ b/zuul.d/tempest-singlenode.yaml
@@ -702,6 +702,9 @@
     parent: neutron-ovn-tempest-ovs-master
     nodeset: devstack-single-node-centos-9-stream
     vars:
+      devstack_localrc:
+        # NOTE(ykarel) remove python3 override with 10-stream switch
+        PYTHON3_VERSION: 3.12
       zuul_copy_output:
         '{{ devstack_base_dir }}/data/ovs': 'logs'
         '{{ devstack_base_dir }}/data/ovn': 'logs'
@@ -721,6 +724,9 @@
       devstack_services:
         br-ex-tcpdump: true
         br-int-flows: true
+      devstack_localrc:
+        # NOTE(ykarel) remove python3 override with 10-stream switch
+        PYTHON3_VERSION: 3.12
       devstack_local_conf:
         test-config:
           "$TEMPEST_CONFIG":
@@ -737,6 +743,9 @@
     vars:
       nslookup_target: 'opendev.org'
       configure_swap_size: 4096
+      devstack_localrc:
+        # NOTE(ykarel) remove python3 override with 10-stream switch
+        PYTHON3_VERSION: 3.12
       devstack_local_conf:
         test-config:
           "$TEMPEST_CONFIG":