diff --git a/octavia/controller/worker/v2/tasks/amphora_driver_tasks.py b/octavia/controller/worker/v2/tasks/amphora_driver_tasks.py
index bffc022bc6..fdf91d23d1 100644
--- a/octavia/controller/worker/v2/tasks/amphora_driver_tasks.py
+++ b/octavia/controller/worker/v2/tasks/amphora_driver_tasks.py
@@ -13,6 +13,7 @@
 # under the License.
 #
 
+import copy
 from cryptography import fernet
 from oslo_config import cfg
 from oslo_log import log as logging
@@ -334,8 +335,9 @@ class AmphoraPostVIPPlug(BaseAmphoraTask):
             **amphorae_network_config[
                 amphora.get(constants.ID)][constants.VRRP_PORT])
         # Required for noop-case
-        vip_arg = amphorae_network_config[amphora.get(
-            constants.ID)][constants.VIP_SUBNET]
+        vip_arg = copy.deepcopy(
+            amphorae_network_config[
+                amphora.get(constants.ID)][constants.VIP_SUBNET])
         if vip_arg:
             host_routes = vip_arg.get('host_routes')
             if host_routes:
diff --git a/octavia/tests/unit/controller/worker/v2/tasks/test_amphora_driver_tasks.py b/octavia/tests/unit/controller/worker/v2/tasks/test_amphora_driver_tasks.py
index 3e80e3df59..28de5af50e 100644
--- a/octavia/tests/unit/controller/worker/v2/tasks/test_amphora_driver_tasks.py
+++ b/octavia/tests/unit/controller/worker/v2/tasks/test_amphora_driver_tasks.py
@@ -630,6 +630,11 @@ class TestAmphoraDriverTasks(base.TestCase):
             self.assertEqual(hr1['destination'], hr2.destination)
             self.assertEqual(hr1['nexthop'], hr2.nexthop)
 
+        self.assertEqual(
+            host_routes,
+            amphorae_net_config_mock[AMP_ID][
+                constants.VIP_SUBNET]['host_routes'])
+
     @mock.patch('octavia.db.repositories.LoadBalancerRepository.update')
     @mock.patch('octavia.db.repositories.LoadBalancerRepository.get')
     def test_amphorae_post_vip_plug(self, mock_lb_get,
diff --git a/releasenotes/notes/fix-host-routes-with-amphorav2-and-persistence-54b99d651a4ee9c4.yaml b/releasenotes/notes/fix-host-routes-with-amphorav2-and-persistence-54b99d651a4ee9c4.yaml
new file mode 100644
index 0000000000..24a859073e
--- /dev/null
+++ b/releasenotes/notes/fix-host-routes-with-amphorav2-and-persistence-54b99d651a4ee9c4.yaml
@@ -0,0 +1,5 @@
+---
+fixes:
+  - |
+    Fix a serialization error when using host_routes in VIP subnets when
+    persistence in the amphorav2 driver is enabled.