diff --git a/jenkins/jobs/devstack-gate.yaml b/jenkins/jobs/devstack-gate.yaml
index 2a2224fe01..e616dcb54e 100644
--- a/jenkins/jobs/devstack-gate.yaml
+++ b/jenkins/jobs/devstack-gate.yaml
@@ -471,7 +471,6 @@
           export DEVSTACK_GATE_NEUTRON=1
           export DEVSTACK_GATE_TEMPEST_FULL=1
           export DEVSTACK_GATE_NEUTRON_DVR=1
-          export DEVSTACK_GATE_TEMPEST_ALLOW_TENANT_ISOLATION=1
           export BRANCH_OVERRIDE={branch-override}
           if [ "$BRANCH_OVERRIDE" != "default" ] ; then
               export OVERRIDE_ZUUL_BRANCH=$BRANCH_OVERRIDE
@@ -503,7 +502,6 @@
           export DEVSTACK_GATE_TEMPEST=1
           export DEVSTACK_GATE_TEMPEST_FULL=1
           export DEVSTACK_GATE_NEUTRON=1
-          export DEVSTACK_GATE_TEMPEST_ALLOW_TENANT_ISOLATION=1
           export BRANCH_OVERRIDE={branch-override}
           if [ "$BRANCH_OVERRIDE" != "default" ] ; then
               export OVERRIDE_ZUUL_BRANCH=$BRANCH_OVERRIDE
@@ -538,7 +536,6 @@
           export DEVSTACK_GATE_TEMPEST_FULL=1
           export DEVSTACK_GATE_POSTGRES=1
           export DEVSTACK_GATE_NEUTRON=1
-          export DEVSTACK_GATE_TEMPEST_ALLOW_TENANT_ISOLATION=1
           export DEVSTACK_GATE_CONFIGDRIVE=0
           export BRANCH_OVERRIDE={branch-override}
           if [ "$BRANCH_OVERRIDE" != "default" ] ; then
@@ -1204,7 +1201,6 @@
           export DEVSTACK_GATE_TEMPEST=1
           export DEVSTACK_GATE_TEMPEST_FULL=1
           export DEVSTACK_GATE_NEUTRON=1
-          export DEVSTACK_GATE_TEMPEST_ALLOW_TENANT_ISOLATION=1
           export DEVSTACK_GATE_KEYSTONE_V3=1
           export BRANCH_OVERRIDE={branch-override}
           if [ "$BRANCH_OVERRIDE" != "default" ] ; then