diff --git a/tox.ini b/tox.ini
index 88ad4865a..d90d8aaf3 100644
--- a/tox.ini
+++ b/tox.ini
@@ -9,7 +9,6 @@ basepython = python3
 install_command = pip install {opts} {packages}
 passenv = *
 setenv =
-  VIRTUAL_ENV={envdir}
   ANSIBLE_CALLBACK_PLUGINS={toxinidir}/callback_plugins
   ANSIBLE_LOOKUP_PLUGINS={toxinidir}/lookup_plugins
   ANSIBLE_LIBRARY={toxinidir}/library
@@ -18,7 +17,6 @@ setenv =
   ANSIBLE_RETRY_FILES_ENABLED=0
   ANSIBLE_STDOUT_CALLBACK=debug
   ANSIBLE_LOG_PATH={envlogdir}/ansible-execution.log
-  VIRTUAL_ENV={envdir}
   # pip: Avoid 2020-01-01 warnings: https://github.com/pypa/pip/issues/6207
   # paramiko CryptographyDeprecationWarning: https://github.com/ansible/ansible/issues/52598
   PYTHONWARNINGS=ignore:DEPRECATION::pip._internal.cli.base_command,ignore::UserWarning